Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
hESC H1
NA
NA

Attributes by original data submitter

Sample

source_name
H1-hESC
treatment
Activin A + Wnt3a 2h
antibody
H3K4me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with H3K27me3 and H3K4me3 antibody. libraries were set up by Berry Genomics BeiJing

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
21932831
Reads aligned (%)
91.0
Duplicates removed (%)
19.1
Number of peaks
25864 (qval < 1E-05)

hg19

Number of total reads
21932831
Reads aligned (%)
90.5
Duplicates removed (%)
19.8
Number of peaks
25775 (qval < 1E-05)

Base call quality data from DBCLS SRA