GSM2157833: DDX11 ChIP-Seq in Control KD rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
DDX11
Cell type
Cell type Class
Lung
Cell type
A549
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma
Attributes by original data submitter
Sample
source_name
A549 cells_anti-DDX11_Control_KD
cell line
A549
knockdown
control KD
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For the ChIP, cells were cross-linked with 1% formaldehyde and chromatin prepared by sonication to obtain DNA fragments between 100 bp and 500 bp. DDX11 bound DNA was then immunoprecipitated using the anti-DDX11 antibody (ab66971, Abcam), decrosslinked and extracted for analysis by sequencing Libraries were prepared using the NEBNext Ultra DNA Library Prep Kit (Illumina)