Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EP300

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
siGATA_p300_estrogen
cell line
MCF-7
cell type
breast cancer cells
stimulated with
E2 at final conc. 100nM for 3 hrs
genotype/variation
GATA knockdown
tranfected with
ON-TARGET plus siRNA J-0033781-09, siGATA3-Custom-1, siGATA3-Custom-2
chip antibody
anti-p300
chip antibody vendor
Santa Cruz

Sequenced DNA Library

library_name
GSM986086: siGATA_p300_E2_r2
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
36492909
Reads aligned (%)
90.9
Duplicates removed (%)
7.3
Number of peaks
27257 (qval < 1E-05)

hg38

Number of total reads
36492909
Reads aligned (%)
92.8
Duplicates removed (%)
6.0
Number of peaks
27349 (qval < 1E-05)

Base call quality data from DBCLS SRA