Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
siNT_ER_estrogen
cell line
MCF-7
cell type
breast cancer cells
stimulated with
E2 at final conc. 100nM for 3 hrs
genotype/variation
negative control
tranfected with
AllStars Negative Control siRNA (Cat nr:1027281, Qiagen)
chip antibody
anti-ERa
chip antibody vendor
Santa Cruz

Sequenced DNA Library

library_name
GSM986063: siNT_ER_E2_r3
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
33806462
Reads aligned (%)
73.2
Duplicates removed (%)
25.9
Number of peaks
77138 (qval < 1E-05)

hg38

Number of total reads
33806462
Reads aligned (%)
74.2
Duplicates removed (%)
25.3
Number of peaks
77212 (qval < 1E-05)

Base call quality data from DBCLS SRA