Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
siNT_ER_estrogen
cell line
MCF-7
cell type
breast cancer cells
stimulated with
E2 at final conc. 100nM for 3 hrs
genotype/variation
negative control
tranfected with
AllStars Negative Control siRNA (Cat nr:1027281, Qiagen)
chip antibody
anti-ERa
chip antibody vendor
Santa Cruz

Sequenced DNA Library

library_name
GSM986059: siNT_ER_E2_r1
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
29991295
Reads aligned (%)
87.6
Duplicates removed (%)
11.0
Number of peaks
23604 (qval < 1E-05)

hg38

Number of total reads
29991295
Reads aligned (%)
89.8
Duplicates removed (%)
9.5
Number of peaks
23616 (qval < 1E-05)

Base call quality data from DBCLS SRA