Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Gonad
Cell type
Testis
MeSH Description
The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Attributes by original data submitter

Sample

source_name
testes
age
4-week-old
genotype
heterozygous
tissue
testes
strain
C57BL/6 (B6) X 129/SvJ hybrid
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Testicular cells were collected by collagenase and trypsin treatment. SimpleChIP Enzymatic Chromatin IP Kit was used to prepare ChIP-seq libraries.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
39431386
Reads aligned (%)
88.0
Duplicates removed (%)
24.3
Number of peaks
300 (qval < 1E-05)

mm9

Number of total reads
39431386
Reads aligned (%)
87.4
Duplicates removed (%)
23.9
Number of peaks
306 (qval < 1E-05)

Base call quality data from DBCLS SRA