GSM2151912: H3K4me3-siATF3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA
Attributes by original data submitter
Sample
source_name
H3K4me3 ChIP-seq
tissue
abdomen skin
cell type
primary dermal fibroblasts
genotype/variation
siATF3
passage
4-6
chip antibody
H3K4m3e (Abcam Cat.No.8580)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin from 5 milions fixed cells was fragmented to a size range of 100–300 bases with a Diagenode Bioruptor. Solubilized chromatin was immunoprecipitated with antibody against H3K27ac, H3K4m3e, H3K27me3 and Polimerase-II. Antibody–chromatin complexes were pulled-down using magnetic beads, washed and then eluted. After cross-link reversal and proteinase K treatment, immunoprecipitated DNA was extracted with phenol-chloroform, ethanol precipitated, and treated with RNase. ChIPed DNA was quantified by fluorometry on the Qubit system (Invitrogen). A total of 10 ng DNA were used for library preparation using NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina, as recommended by the manufacturer. NEBNext ChIP-seq Library kit (New England Biolabs)