Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
Naive T cells
NA
NA

Attributes by original data submitter

Sample

source_name
spleen + lymph nodes
mouse strain
C57Bl.6
cell type
naive CD4 purified cells
chip antibodies
H3K27Ac (Diagenode, C15410196)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA was harvested using BioRad total RNA extraction kit with DNaseI treatment. For ChIP cells were fixed with 0.8% formaldehyde for 8 min at RT and DNA was processed and sonicated according to the protocol from Covaris using Covaris S220 sonicator for 4-5 min. RNA libraries for pair-end read sequencing were prepared by using standard Illumina protocols. ChIP libraries were prepared by using ThruPLEX DNA kit (Rubicon).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
9068091
Reads aligned (%)
98.7
Duplicates removed (%)
11.6
Number of peaks
8634 (qval < 1E-05)

mm9

Number of total reads
9068091
Reads aligned (%)
98.5
Duplicates removed (%)
11.8
Number of peaks
8561 (qval < 1E-05)

Base call quality data from DBCLS SRA