Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Lung
Cell type
NCI-H1299
Primary Tissue
Lung
Tissue Diagnosis
Carcinoma Non-Small Cell

Attributes by original data submitter

Sample

source_name
NCI-H1299-EGFP/NEO
treatment
SB939
antibody
H3K4me3

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
total RNA was isolated using the Rneasy kit (Qiagen); genomic DNA was isolated using the DNeasy blood & tissue kit (Qiagen) For CAGE, ChIP-, and WGB-sequencing the CAGE™ Preparation Kit (DNAFORM.jp), NEBNext Ultra Prep Kit for Illumina (NEB), and EpiTect Bisulfite (Qiagen) + TruSeq DNA PCR-Free LT Sample Prep Kit (Illumina) were used, respectively

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
30183382
Reads aligned (%)
99.2
Duplicates removed (%)
31.6
Number of peaks
26834 (qval < 1E-05)

hg19

Number of total reads
30183382
Reads aligned (%)
99.1
Duplicates removed (%)
31.7
Number of peaks
26689 (qval < 1E-05)

Base call quality data from DBCLS SRA