Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AML1-ETO

Cell type

Cell type Class
Blood
Cell type
Kasumi-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
Kasumi-1 cell line
transduction
KDMEIS2
cell type
leukemic cell line derived from peripheral blood (contains t(8;21) chromosome translocation)
passages
10-15
cell line
Kasumi-1
chip antibody
AML1-ETO (Diagenode, Cat. C15310197; Lot A706-001)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was fixed in 1% formaldehyde for 10 minutes, follwed by shearing them to 200-700bp using a Bioruptor (Diagenode). Samples were immunoprecipitated using AML1-ETO (Diagenode, Cat. C15310197; Lot A706-001) antibody. Sequencing samples were prepared according to the manufacturer's protocol (Illumina). End repair was performed using the precipitated DNA using Klenow and T4 PNK. A 3’ protruding A base was generated using Taq polymerase and adapters were ligated. The DNA was loaded on gel and a band corresponding to ~300 bp (ChIP fragment + adapters) was excised. The DNA was isolated, amplified by PCR and used for cluster generation. ChIP-seq libraries were further processed according to Illumina's standard protocol.

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg38

Number of total reads
52611509
Reads aligned (%)
98.2
Duplicates removed (%)
66.9
Number of peaks
6804 (qval < 1E-05)

hg19

Number of total reads
52611509
Reads aligned (%)
97.2
Duplicates removed (%)
68.8
Number of peaks
6779 (qval < 1E-05)

Base call quality data from DBCLS SRA