Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me3

Cell type

Cell type Class
Blood
Cell type
Granulocytic-monocytic progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
L-GMP transformed with MLL-AF9
cell type
L-GMP transformed with MLL-AF9
genotype
Jmjd2a/b/cf/f;Rosa26::CreERT2
strain background
C57BL/6
chip antibody
anti-H3K36me3 (Cell Signaling Technology, #4909)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIPSeq libraries were made using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® according to the manufactures protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
48393236
Reads aligned (%)
96.7
Duplicates removed (%)
7.7
Number of peaks
454 (qval < 1E-05)

mm9

Number of total reads
48393236
Reads aligned (%)
96.5
Duplicates removed (%)
7.8
Number of peaks
479 (qval < 1E-05)

Base call quality data from DBCLS SRA