Antigen

Antigen Class

TFs and others

Antigen

Kdm4a

Cell type

Cell type Class

Blood

Cell type

Granulocytic-monocytic progenitors

NA

NA

Sample

source_name

L-GMP transformed with MLL-AF9

cell type

L-GMP transformed with MLL-AF9

genotype

Jmjd2a/b/cf/f;Rosa26::CreERT2

strain background

C57BL/6

chip antibody

anti-Jmjd2a (Cell Signaling Technology, #5328)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIPSeq libraries were made using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® according to the manufactures protocol

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

21092937

Reads aligned (%)

94.2

Duplicates removed (%)

14.4

Number of peaks

396 (qval < 1E-05)

mm9

Number of total reads

21092937

Reads aligned (%)

94.0

Duplicates removed (%)

14.5

Number of peaks

428 (qval < 1E-05)