Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Kdm4a

Cell type

Cell type Class
Blood
Cell type
Granulocytic-monocytic progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
L-GMP transformed with MLL-AF9
cell type
L-GMP transformed with MLL-AF9
genotype
Jmjd2a/b/cf/f;Rosa26::CreERT2
strain background
C57BL/6
chip antibody
anti-Jmjd2a (Cell Signaling Technology, #5328)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIPSeq libraries were made using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® according to the manufactures protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
21092937
Reads aligned (%)
80.7
Duplicates removed (%)
14.4
Number of peaks
396 (qval < 1E-05)

mm9

Number of total reads
21092937
Reads aligned (%)
80.4
Duplicates removed (%)
14.5
Number of peaks
428 (qval < 1E-05)

Base call quality data from DBCLS SRA