Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CTCF

Cell type

Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
human monocytic cell line THP-1
treatment
100 nM 1,25(OH)2D3
treatment time
24 h
antibody
CTCF (Millipore 07-729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
proteins were crosslinked with formaldehyde to DNA, then VDR-bound fragments were pulled down by chromatin immunoprecipitation Standard protocol as suggested by the manufacturer for ChIP-seq libraries as utilized at the EMBL Gene Core in Heidelberg, Germany

Sequencing Platform

instrument_model
Illumina Genome Analyzer II

hg19

Number of total reads
35515932
Reads aligned (%)
71.6
Duplicates removed (%)
26.5
Number of peaks
29126 (qval < 1E-05)

hg38

Number of total reads
35515932
Reads aligned (%)
73.7
Duplicates removed (%)
25.0
Number of peaks
29313 (qval < 1E-05)

Base call quality data from DBCLS SRA