Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Blood

Cell type

MOLM-13

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

MOLM13

cell line

MOLM13

treatment

Y78A mutation

antibody

Flag M2, Sigma F3165

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq were performed following the protocol: Using ChIP-seq technology to generate high-resolution profiles of histone modifications. O'Geen H, Echipare L, Farnham PJ. Methods Mol Biol. 2011;791:265-86. doi: 10.1007/978-1-61779-316-5_20. Sequencing libraries were prepared using the Illumina TruSeq DNA Sample Preparation Kit according to the manufacturer's protocol. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250-450 bp (insert plus adaptor) were band isolated from an agarose gel. DNA fragments were sequenced using paired-end sequencing technology on Illumina HiSeq 2000 platform.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

54928591

Reads aligned (%)

86.3

Duplicates removed (%)

2.2

Number of peaks

1249 (qval < 1E-05)

hg19

Number of total reads

54928591

Reads aligned (%)

85.5

Duplicates removed (%)

3.9

Number of peaks

1410 (qval < 1E-05)