Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CDK9

Cell type

Cell type Class
Blood
Cell type
MOLM-13
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MOLM13
cell line
MOLM13
treatment
Control
antibody
Santa Cruz, sc-484

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq were performed following the protocol: Using ChIP-seq technology to generate high-resolution profiles of histone modifications. O'Geen H, Echipare L, Farnham PJ. Methods Mol Biol. 2011;791:265-86. doi: 10.1007/978-1-61779-316-5_20. Sequencing libraries were prepared using the Illumina TruSeq DNA Sample Preparation Kit according to the manufacturer's protocol. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250-450 bp (insert plus adaptor) were band isolated from an agarose gel. DNA fragments were sequenced using paired-end sequencing technology on Illumina HiSeq 2000 platform.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
49885603
Reads aligned (%)
89.5
Duplicates removed (%)
3.8
Number of peaks
1331 (qval < 1E-05)

hg38

Number of total reads
49885603
Reads aligned (%)
91.9
Duplicates removed (%)
2.2
Number of peaks
1116 (qval < 1E-05)

Base call quality data from DBCLS SRA