Doxycycline (DOX) treated BCR-ABL1-transformed primary B cell precursors that carry a DOX-inducible CEBPA vector. Cells were enriched for CD11B+ myeloid phenotype by MACS. Cells are succesfully transformed cells.
Cells were enriched for CD11B and dead cells removed using MACS, cells formaldehyde treated and pellets snap frozen. Pellets were resuspended in sonication buffer, sonicated and lysed in RIPA buffer before performing ChIP with the appropriate antibody. ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set and NEBNEXT Multiplex Oligos for Illumina.