Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
B cell precursors
NA
NA

Attributes by original data submitter

Sample

source_name
Doxycycline (DOX) treated BCR-ABL1-transformed primary B cell precursors that carry a DOX-inducible CEBPA vector. Cells were enriched for CD11B+ myeloid phenotype by MACS. Cells are succesfully transformed cells.
genotype
C57BL/6
vector transduced
MSCV BCR-ABL1-p210; pRetroX-TRE3G-Cebpa; pRetroX-Tet3G
antibody
none
treatment
Doxycycline treated

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were enriched for CD11B and dead cells removed using MACS, cells formaldehyde treated and pellets snap frozen. Pellets were resuspended in sonication buffer, sonicated and lysed in RIPA buffer before performing ChIP with the appropriate antibody. ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set and NEBNEXT Multiplex Oligos for Illumina.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
39218647
Reads aligned (%)
92.2
Duplicates removed (%)
9.3
Number of peaks
4674 (qval < 1E-05)

mm9

Number of total reads
39218647
Reads aligned (%)
92.1
Duplicates removed (%)
9.3
Number of peaks
4631 (qval < 1E-05)

Base call quality data from DBCLS SRA