GSM2133284: BCR-ABL1 iso ChIPseq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
B cell precursors
NA
NA
Attributes by original data submitter
Sample
source_name
BCR-ABL1 expressing primary B cell precursors (D7-9 post transduction)
genotype
53BP1-/- C57BL/6
vector transduced
BCR-ABL1p210 MIGR1
antibody
polyclonal rabbit IgG (sc2027, Santa Cruz Biotechnologies)
treatment
none
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Dead cells were removed, cells formaldehyde treated and pellets snap frozen. Pellets were resuspended in sonication buffer, sonicated and lysed in RIPA buffer before performing ChIP with the appropriate antibody. ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set and NEBNEXT Multiplex Oligos for Illumina.