Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
U-266
Primary Tissue
Blood
Tissue Diagnosis
Multiple Myeloma

Attributes by original data submitter

Sample

source_name
Multiple Myeloma
cell line
U266
input
N/A
treatment
none
barcode
ATCACG
antibody
N/A
antibody maker
N/A
antibody catalog number
N/A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and chromatin-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
78843707
Reads aligned (%)
93.8
Duplicates removed (%)
16.1
Number of peaks
18787 (qval < 1E-05)

hg19

Number of total reads
78843707
Reads aligned (%)
93.5
Duplicates removed (%)
16.6
Number of peaks
19435 (qval < 1E-05)

Base call quality data from DBCLS SRA