Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

U-266

Primary Tissue

Blood

Tissue Diagnosis

Multiple Myeloma

Sample

source_name

Multiple Myeloma

cell line

U266

input

N/A

treatment

none

barcode

N/A

antibody

N/A

antibody maker

N/A

antibody catalog number

N/A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and chromatin-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

106004321

Reads aligned (%)

96.8

Duplicates removed (%)

5.6

Number of peaks

5570 (qval < 1E-05)

hg19

Number of total reads

106004321

Reads aligned (%)

95.9

Duplicates removed (%)

7.0

Number of peaks

4996 (qval < 1E-05)