Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
U-266
Primary Tissue
Blood
Tissue Diagnosis
Multiple Myeloma

Attributes by original data submitter

Sample

source_name
Multiple Myeloma
cell line
U266
input
U266_WCE_2
treatment
none
barcode
CGATGT
antibody
anti H3K27AC
antibody maker
Abcam
antibody catalog number
AB4729

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and chromatin-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
68357881
Reads aligned (%)
96.3
Duplicates removed (%)
15.8
Number of peaks
28469 (qval < 1E-05)

hg19

Number of total reads
68357881
Reads aligned (%)
96.1
Duplicates removed (%)
16.4
Number of peaks
28483 (qval < 1E-05)

Base call quality data from DBCLS SRA