Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
E2F1

Cell type

Cell type Class
Blood
Cell type
U-266
Primary Tissue
Blood
Tissue Diagnosis
Multiple Myeloma

Attributes by original data submitter

Sample

source_name
Multiple Myeloma
cell line
U266
input
U266_WCE
treatment
none
barcode
N/A
antibody
anti E2F1
antibody maker
Cell Signaling
antibody catalog number
Cell Signaling #3742

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and chromatin-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
32303813
Reads aligned (%)
90.2
Duplicates removed (%)
12.2
Number of peaks
3249 (qval < 1E-05)

hg19

Number of total reads
32303813
Reads aligned (%)
89.5
Duplicates removed (%)
13.6
Number of peaks
3247 (qval < 1E-05)

Base call quality data from DBCLS SRA