Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D breast cancer cells
cell type
T47D
chip antibody
H3K4me3 Abcam (Ab8580)
treatment
MK2206

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked, lysed in SDS lysis buffer, and sonicated. ThruPLEX-FD Prep kit, Rubicon Genomics

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
54738766
Reads aligned (%)
86.2
Duplicates removed (%)
11.2
Number of peaks
29206 (qval < 1E-05)

hg19

Number of total reads
54738766
Reads aligned (%)
85.4
Duplicates removed (%)
12.4
Number of peaks
28841 (qval < 1E-05)

Base call quality data from DBCLS SRA