Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ETV1

Cell type

Cell type Class
Epidermis
Cell type
COLO 800
Primary Tissue
Skin
Tissue Diagnosis
Melanoma

Attributes by original data submitter

Sample

source_name
Colo800 Human melanoma Cells
cell line
Colo800
antibody
ETV1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was isolated and sheared. For ETV1 IP, chromatin was IP'd using anti-ETV1 antibody (abcam ab81086) and magnetic protein A/G beads.The immunoprecipate was washed and eluted using standing conditions. RNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
30018497
Reads aligned (%)
91.1
Duplicates removed (%)
3.3
Number of peaks
21345 (qval < 1E-05)

hg38

Number of total reads
30018497
Reads aligned (%)
92.7
Duplicates removed (%)
2.2
Number of peaks
21399 (qval < 1E-05)

Base call quality data from DBCLS SRA