Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ETV1

Cell type

Cell type Class
Epidermis
Cell type
A-375
Primary Tissue
Skin
Tissue Diagnosis
Melanoma

Attributes by original data submitter

Sample

source_name
A375 Human melanoma Cells
cell line
A375
antibody
ETV1

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was isolated and sheared. For ETV1 IP, chromatin was IP'd using anti-ETV1 antibody (abcam ab81086) and magnetic protein A/G beads.The immunoprecipate was washed and eluted using standing conditions. RNA libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
36649318
Reads aligned (%)
89.8
Duplicates removed (%)
3.1
Number of peaks
15632 (qval < 1E-05)

hg38

Number of total reads
36649318
Reads aligned (%)
91.3
Duplicates removed (%)
2.1
Number of peaks
15728 (qval < 1E-05)

Base call quality data from DBCLS SRA