Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
Breast cancer cells
tissue type
ER+/PRA+/PRB- T47D cell models
er/pr status
ER+/PRA+/PRB-
drug treatment
10 nM R5020
hormone exposure time
45 minutes
chip antibody
anti-ER (SC biotech HC-20, cat# SC-543, lot# F0515) plus anti-rabbit IgG Control (cat# sc-2027, lot# E2914 or H2615)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Hormone-treated were harvested and ChIP was performed as per the directions provided in materials and method (ChIP and ChIP-sequencing; reChIP and reChIP-sequencing) ChIP-seq and reChIP-seq libraries were prepared using Kapa ChIP-seq kits. Details of library preparation strategy are provided in the materials and methods section (ChIP and ChIP-seq; reChIP and reChIP-seq) of the paper.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
26410365
Reads aligned (%)
1.4
Duplicates removed (%)
96.5
Number of peaks
24 (qval < 1E-05)

hg38

Number of total reads
26410365
Reads aligned (%)
1.7
Duplicates removed (%)
95.8
Number of peaks
16 (qval < 1E-05)

Base call quality data from DBCLS SRA