Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AR

Cell type

Cell type Class
Prostate
Cell type
LNCAP
Primary Tissue
Prostate
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
abl_GSK treated
cell line
LNCaP-abl (abl)
cell types
castration resitant prostate cancer cells
cell phenotype
AR-positive, EZH2 inhibitor-sensitive
passages
61-64
treated with
GSK126
chip antibody
AR Antibody (H-280) (Santa Cruz, sc-13062)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Prostate cancer cells were crosslinked with 1% formaldehyde and lysed in RIPA buffer with 0.3 M NaCl. ChIP DNA was purified using PCR Purification Kit (Qiagen) and then quantified by Quant-iTTM dsDNA HS Assay Kit (Invitrogen). ChIP-Seq libraries were prepared using ThruPLEX-FD Prep Kit (Rubicon Genomics) according to the manufacturer's protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
35982580
Reads aligned (%)
87.9
Duplicates removed (%)
6.9
Number of peaks
1019 (qval < 1E-05)

hg38

Number of total reads
35982580
Reads aligned (%)
89.5
Duplicates removed (%)
5.8
Number of peaks
950 (qval < 1E-05)

Base call quality data from DBCLS SRA