Antigen

Antigen Class

TFs and others

Antigen

AR

Cell type

Cell type Class

Prostate

Cell type

LNCAP

Primary Tissue

Prostate

Tissue Diagnosis

Carcinoma

Sample

source_name

abl_DMSO treated

cell line

LNCaP-abl (abl)

cell types

castration resitant prostate cancer cells

cell phenotype

AR-positive, EZH2 inhibitor-sensitive

passages

61-64

treated with

vehicle (DMSO)

chip antibody

AR Antibody (H-280) (Santa Cruz, sc-13062)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Prostate cancer cells were crosslinked with 1% formaldehyde and lysed in RIPA buffer with 0.3 M NaCl. ChIP DNA was purified using PCR Purification Kit (Qiagen) and then quantified by Quant-iTTM dsDNA HS Assay Kit (Invitrogen). ChIP-Seq libraries were prepared using ThruPLEX-FD Prep Kit (Rubicon Genomics) according to the manufacturer's protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

31310316

Reads aligned (%)

96.6

Duplicates removed (%)

17.5

Number of peaks

9399 (qval < 1E-05)

hg19

Number of total reads

31310316

Reads aligned (%)

95.8

Duplicates removed (%)

19.1

Number of peaks

9334 (qval < 1E-05)