Antigen

Antigen Class

TFs and others

Antigen

MYCN

Cell type

Cell type Class

Neural

Cell type

Neuroblastoma

MeSH Description

A common neoplasm of early childhood arising from neural crest cells in the sympathetic nervous system, and characterized by diverse clinical behavior, ranging from spontaneous remission to rapid metastatic progression and death. This tumor is the most common intraabdominal malignancy of childhood, but it may also arise from thorax, neck, or rarely occur in the central nervous system. Histologic features include uniform round cells with hyperchromatic nuclei arranged in nests and separated by fibrovascular septa. Neuroblastomas may be associated with the opsoclonus-myoclonus syndrome. (From DeVita et al., Cancer: Principles and Practice of Oncology, 5th ed, pp2099-2101; Curr Opin Oncol 1998 Jan;10(1):43-51)

Sample

source_name

SHEP-21N_Neuroblastoma

cell line

SHEP-21N

cell type

neuroblastoma cell line

growth condition

10% Tet-Free FBS, RPMI

treated with

0.2 ug/mL DOX for 2HR

chip antibody

MYCN

chip antibody vendor

abcam

input

SHEP21_2HR_INPUT

barcode

GCCAAT

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

87578025

Reads aligned (%)

95.5

Duplicates removed (%)

31.7

Number of peaks

20012 (qval < 1E-05)

hg19

Number of total reads

87578025

Reads aligned (%)

95.1

Duplicates removed (%)

32.8

Number of peaks

19724 (qval < 1E-05)