Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYCN

Cell type

Cell type Class
Neural
Cell type
NGP
NA
NA

Attributes by original data submitter

Sample

source_name
NGP_Neuroblastoma
cell line
NGP
cell type
neuroblastoma cell line
growth condition
10% FBS, DMEM
treated with
None
chip antibody
MYCN
chip antibody vendor
abcam
input
NGP_INPUT
barcode
TAGTCTTG

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
46601433
Reads aligned (%)
90.9
Duplicates removed (%)
7.2
Number of peaks
9951 (qval < 1E-05)

hg38

Number of total reads
46601433
Reads aligned (%)
92.2
Duplicates removed (%)
6.4
Number of peaks
9997 (qval < 1E-05)

Base call quality data from DBCLS SRA