Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TWIST1

Cell type

Cell type Class
Neural
Cell type
BE(2)-C
Primary Tissue
Brain
Site of Extraction
Bone Marrow
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
BE(2)-C_Neuroblastoma
cell line
BE(2)-C
cell type
neuroblastoma cell line
growth condition
10% FBS, DMEM
treated with
None
chip antibody
TWIST
chip antibody vendor
abcam
input
BE2C_INPUT
barcode
TAACGCTG

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
53123407
Reads aligned (%)
28.6
Duplicates removed (%)
70.7
Number of peaks
24801 (qval < 1E-05)

hg38

Number of total reads
53123407
Reads aligned (%)
29.5
Duplicates removed (%)
69.9
Number of peaks
24852 (qval < 1E-05)

Base call quality data from DBCLS SRA