Antigen

Antigen Class

TFs and others

Antigen

MYCN

Cell type

Cell type Class

Neural

Cell type

BE(2)-C

Primary Tissue

Brain

Site of Extraction

Bone Marrow

Tissue Diagnosis

Neuroblastoma

Sample

source_name

BE(2)-C_Neuroblastoma

cell line

BE(2)-C

cell type

neuroblastoma cell line

growth condition

10% FBS, DMEM

treated with

None

chip antibody

MYCN

chip antibody vendor

abcam

input

BE2C_INPUT

barcode

GTCCGC

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

40389274

Reads aligned (%)

94.6

Duplicates removed (%)

6.8

Number of peaks

32544 (qval < 1E-05)

hg19

Number of total reads

40389274

Reads aligned (%)

94.0

Duplicates removed (%)

7.6

Number of peaks

32363 (qval < 1E-05)