Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
BE(2)-C
Primary Tissue
Brain
Site of Extraction
Bone Marrow
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
BE(2)-C_Neuroblastoma
cell line
BE(2)-C
cell type
neuroblastoma cell line
growth condition
10% FBS, DMEM
treated with
None
chip antibody
NA
chip antibody vendor
NA
input
NA
barcode
ACAGTG

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
28842039
Reads aligned (%)
97.6
Duplicates removed (%)
3.9
Number of peaks
634 (qval < 1E-05)

hg19

Number of total reads
28842039
Reads aligned (%)
96.8
Duplicates removed (%)
4.7
Number of peaks
850 (qval < 1E-05)

Base call quality data from DBCLS SRA