SRX1690203
GSM2113519: BE2C H3K4ME3; Homo sapiens; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Neural
Cell type
BE(2)-C
Primary Tissue
Brain
Site of Extraction
Bone Marrow
Tissue Diagnosis
Neuroblastoma

Attributes by original data submitter

Sample

source_name
BE(2)-C_Neuroblastoma
cell line
BE(2)-C
cell type
neuroblastoma cell line
growth condition
10% FBS, DMEM
treated with
None
chip antibody
H3K4me3
chip antibody vendor
EMD Millipore
input
BE2C_INPUT
barcode
CAGATC

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using the Rubicon Thruplex FD library preparation kit (cat#: 400427) per manufacturers instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

Where can I get the processing logs?

Read processing pipeline log

hg38

Number of total reads
29617296
Reads aligned (%)
97.9
Duplicates removed (%)
21.6
Number of peaks
41461 (qval < 1E-05)

hg19

Number of total reads
29617296
Reads aligned (%)
97.7
Duplicates removed (%)
21.8
Number of peaks
41357 (qval < 1E-05)

Base call quality data from DBCLS SRA