GSM2112609: H3K4me3-Dox-plus; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Embryonic stem cells
cell line
SEES-3
chip antibody
H3K4me3 (Millipore, 07-473)
treatment
Doxycycline (1 ug/ml)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The ChIPed DNA libraries were constructed using NEBNext ChIP-Seq Library Prep Kit for Illumina (NEB).