Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
Breast cancer cells
tissue type
ER+/PR+ cell models
cell line
T47D
er/pr status
ER+/PR+
drug treatment
10nM Estradiol + 10 nM R5020

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Hormone-treated were harvested and ChIP was performed as per the directions provided in materials and method (ChIP and ChIP-sequencing; reChIP and reChIP-sequencing) ChIP-seq and reChIP-seq libraries were prepared using Kapa ChIP-seq kits. Details of library preparation strategy are provided in the materials and methods section (ChIP and ChIP-seq; reChIP and reChIP-seq) of the paper.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
36918476
Reads aligned (%)
99.3
Duplicates removed (%)
2.7
Number of peaks
984 (qval < 1E-05)

hg19

Number of total reads
36918476
Reads aligned (%)
98.4
Duplicates removed (%)
4.1
Number of peaks
1023 (qval < 1E-05)

Base call quality data from DBCLS SRA