Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Thymocytes

MeSH Description

HEMATOPOIETIC PROGENITOR CELLS that have migrated to the THYMUS where they differentiate into T-LYMPHOCYTES. Thymocytes are classified into maturational stages based on the expression of CELL SURFACE ANTIGENS.

Sample

source_name

thymus

cell type

DP thymocytes

genotype

wild type

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit (Kapa Biosystems, USA) according to the manufacture’s protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

11225735

Reads aligned (%)

87.9

Duplicates removed (%)

47.1

Number of peaks

229 (qval < 1E-05)

mm9

Number of total reads

11225735

Reads aligned (%)

87.2

Duplicates removed (%)

47.2

Number of peaks

244 (qval < 1E-05)