Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

Oocytes

MeSH Description

Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).

Sample

source_name

P12 oocyte

chip antibody

None

cell type

P12 oocyte

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP chromatin was preapred from whole cell lysates of cross-linked cells. Lysates were sonicated and chromatin complexes were isolated with antibodies coupled to magnetic beads. RNAs were extracted from whole cell lysates by following QIAGEN RNeasy Micro handbook. Genomic DNA for WGBS was obtained from lysates from 100-500 P12 or P15 oocytes. ChIP-seq libraries were prepared according to Rubicon Genomics THRUPlex kit with minor modifications. RNA-seq library preparation was carried out according to TruSeq library preparation. MethylC-Seq libraris were prepared with modified library protocol from Wang, L., et al., 2014 Cell.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

38148943

Reads aligned (%)

97.3

Duplicates removed (%)

39.6

Number of peaks

184 (qval < 1E-05)

mm9

Number of total reads

38148943

Reads aligned (%)

97.2

Duplicates removed (%)

39.6

Number of peaks

147 (qval < 1E-05)