Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Gonad

Cell type

JHOS-4

Primary Tissue

Ovary

Tissue Diagnosis

Adenocarcinoma Cystic

Sample

source_name

JHOS4

cell line

JHOS4

cell line type

High grade serous ovarian carcinoma

antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with PAX8 antibody (Novus, Product #NBP1-32440, Littleton, CO). After repairing the DNA ends, sequencing adaptors (Illumina, San Diego, CA) were ligated to the purified chromatin, and the DNA was amplified for sequencing

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

993488

Reads aligned (%)

82.5

Duplicates removed (%)

10.7

Number of peaks

391 (qval < 1E-05)

hg19

Number of total reads

993488

Reads aligned (%)

61.9

Duplicates removed (%)

27.6

Number of peaks

556 (qval < 1E-05)