Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PAX8

Cell type

Cell type Class
Gonad
Cell type
JHOS-4
Primary Tissue
Ovary
Tissue Diagnosis
Adenocarcinoma Cystic

Attributes by original data submitter

Sample

source_name
JHOS4
cell line
JHOS4
cell line type
High grade serous ovarian carcinoma
antibody
PAX8 antibody (Novus, Product #NBP1-32440, Littleton, CO)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with PAX8 antibody (Novus, Product #NBP1-32440, Littleton, CO). After repairing the DNA ends, sequencing adaptors (Illumina, San Diego, CA) were ligated to the purified chromatin, and the DNA was amplified for sequencing

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
1134715
Reads aligned (%)
42.2
Duplicates removed (%)
29.3
Number of peaks
596 (qval < 1E-05)

hg38

Number of total reads
1134715
Reads aligned (%)
69.6
Duplicates removed (%)
10.2
Number of peaks
432 (qval < 1E-05)

Base call quality data from DBCLS SRA