Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
PAX8

Cell type

Cell type Class
Gonad
Cell type
KURAMOCHI
Primary Tissue
Ovary
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
KURAMOCHI
cell line
KURAMOCHI
cell line type
High grade serous ovarian carcinoma
antibody
PAX8 antibody (Novus, Product #NBP1-32440, Littleton, CO)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with PAX8 antibody (Novus, Product #NBP1-32440, Littleton, CO). After repairing the DNA ends, sequencing adaptors (Illumina, San Diego, CA) were ligated to the purified chromatin, and the DNA was amplified for sequencing

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
794428
Reads aligned (%)
45.9
Duplicates removed (%)
23.8
Number of peaks
519 (qval < 1E-05)

hg38

Number of total reads
794428
Reads aligned (%)
72.2
Duplicates removed (%)
7.8
Number of peaks
393 (qval < 1E-05)

Base call quality data from DBCLS SRA