Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Uterus

Cell type

FT194

NA

NA

Sample

source_name

FT194

cell line

FT194

cell line type

Immortalized fallopian tube secretory cell

antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with PAX8 antibody (Novus, Product #NBP1-32440, Littleton, CO). After repairing the DNA ends, sequencing adaptors (Illumina, San Diego, CA) were ligated to the purified chromatin, and the DNA was amplified for sequencing

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

750130

Reads aligned (%)

83.6

Duplicates removed (%)

7.0

Number of peaks

338 (qval < 1E-05)

hg19

Number of total reads

750130

Reads aligned (%)

64.6

Duplicates removed (%)

23.2

Number of peaks

513 (qval < 1E-05)