Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Uterus
Cell type
FT33
NA
NA

Attributes by original data submitter

Sample

source_name
FT33
cell line
FT33
cell line type
Immortalized fallopian tube secretory cell
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with PAX8 antibody (Novus, Product #NBP1-32440, Littleton, CO). After repairing the DNA ends, sequencing adaptors (Illumina, San Diego, CA) were ligated to the purified chromatin, and the DNA was amplified for sequencing

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
587868
Reads aligned (%)
83.6
Duplicates removed (%)
6.7
Number of peaks
305 (qval < 1E-05)

hg19

Number of total reads
587868
Reads aligned (%)
63.6
Duplicates removed (%)
21.5
Number of peaks
450 (qval < 1E-05)

Base call quality data from DBCLS SRA