Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GATAD2A

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa FRT-TO GFP-GATAD2A
antibody
GFP (Abcam, ab290)
cross-linking
45 min DSG, 10 min Formaldehyde
down sampling
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepped using 5ng of ChIP material and the Kapa Hyper Prep Kit for Illumina Sequencing (Kapa Biosystems) according to manufacturer's protocol.

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
23930052
Reads aligned (%)
46.0
Duplicates removed (%)
52.3
Number of peaks
4924 (qval < 1E-05)

hg38

Number of total reads
23930052
Reads aligned (%)
46.6
Duplicates removed (%)
51.8
Number of peaks
4873 (qval < 1E-05)

Base call quality data from DBCLS SRA