Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ZMYND8

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa Kyoto CRISPR ZMYND8 KO
antibody
ZMYND8 (HPA020949)
cross-linking
45 min DSG, 10 min Formaldehyde
down sampling
0.56

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepped using 5ng of ChIP material and the Kapa Hyper Prep Kit for Illumina Sequencing (Kapa Biosystems) according to manufacturer's protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
55399952
Reads aligned (%)
89.4
Duplicates removed (%)
8.3
Number of peaks
1324 (qval < 1E-05)

hg38

Number of total reads
55399952
Reads aligned (%)
91.6
Duplicates removed (%)
6.8
Number of peaks
1405 (qval < 1E-05)

Base call quality data from DBCLS SRA