Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Spleen
Cell type
Spleen
MeSH Description
An encapsulated lymphatic organ through which venous blood filters.

Attributes by original data submitter

Sample

source_name
splenic B220+ lymphocytes
cell type
lymphoma cells
genotype/variation
Crebbp knock-down
chip antibody
NA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was sheared to 200-400bp, incubated with H3K27ac antibody (abcam 4729) overnight at 4C on a rocker, antibody chromatin complex was captured by ProteinA agarose beads, washed with low salt, high salt, LiCl, and TE buffer, eluted and then de-crosslinked at 65C with 0.3M NaCl final for at least 6 hr. Final chormatin was purified using Qiagen PCR purification columns. Libraries were prepared using the Illumina Truseq ChIPseq Library preparation Kit following the manufacture’s instructions (Illumina, CA).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
22478461
Reads aligned (%)
96.0
Duplicates removed (%)
14.3
Number of peaks
484 (qval < 1E-05)

mm9

Number of total reads
22478461
Reads aligned (%)
95.8
Duplicates removed (%)
14.4
Number of peaks
498 (qval < 1E-05)

Base call quality data from DBCLS SRA