Antigen

Antigen Class

TFs and others

Antigen

TRIM25

Cell type

Cell type Class

Breast

Cell type

Breast cancer cells

NA

NA

Sample

source_name

breast cancer

cell type

breast cancer cell line

subtype

triple negative breast cancer

chip antibody

EFP (BD, 610570, lot 5061873)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed using the EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit (Millipore) according to manufacturers. instructions. Libraries were prepared using KAPA HTP library preparation kit (KK8234).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

41389724

Reads aligned (%)

92.3

Duplicates removed (%)

26.4

Number of peaks

32066 (qval < 1E-05)

hg19

Number of total reads

41389724

Reads aligned (%)

91.4

Duplicates removed (%)

27.2

Number of peaks

31473 (qval < 1E-05)