Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TRIM25

Cell type

Cell type Class
Breast
Cell type
Breast cancer cells
NA
NA

Attributes by original data submitter

Sample

source_name
breast cancer
cell type
breast cancer cell line
subtype
triple negative breast cancer
chip antibody
EFP (BD, 610570, lot 5061873)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was performed using the EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit (Millipore) according to manufacturers. instructions. Libraries were prepared using KAPA HTP library preparation kit (KK8234).

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
41389724
Reads aligned (%)
133.2
Duplicates removed (%)
27.2
Number of peaks
31473 (qval < 1E-05)

hg38

Number of total reads
41389724
Reads aligned (%)
135.9
Duplicates removed (%)
26.4
Number of peaks
32066 (qval < 1E-05)

Base call quality data from DBCLS SRA