Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

EpiLC

NA

NA

Sample

source_name

E14 mouse embryonic stem cells

cell-type

wild-type Epiblast-like

antibody

Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9735

sample type

Day 7 of Activin/FGF2 treatment

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were obtained from cross-linked nuclei, and chromatin was immunopreciptated with antibodies against H3K27me3

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

42228291

Reads aligned (%)

98.4

Duplicates removed (%)

12.0

Number of peaks

644 (qval < 1E-05)

mm9

Number of total reads

42228291

Reads aligned (%)

98.2

Duplicates removed (%)

12.0

Number of peaks

640 (qval < 1E-05)