GSM2095926: Input, replicate 2; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
Kasumi-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
acute myeloid leukemia
cell line
acute myeloid leukemia cell line Kasumi-1
antibody
none
treatment
NR4A1 mRNA electroporation
genotype/variation
NR4A1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed by Dounce homogenization and chromatin was sheared to an average length of 300-500bp. 30ug aliquots of lysates were pre-cleared with Protein A agarose beads and incubated with 2ug NR4A antibody (sc-990, Santa Cruz Biotechnology) overnight at 4ºC. ChIP and input DNA were amplified using the Illumina ChIP-Seq DNA Sample Prep Kit.