GSM2094985: FLAG ChIP-seq negative control; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
MEL
Tissue
Blood
Lineage
cellLine
Description
Leukemia (K562 analog)
Attributes by original data submitter
Sample
source_name
MEL-FLAG Control Cells
cell type
MEL cells
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was extracted by phenol/choloroform method. 5hmC enrichment was performed using a previously described procedure with an improved selective chemical labeling method (Song et al, Nature Biotechnology, 2011). DNA libraries were generated following the Illumina protocol for “Preparing Samples for ChIP Sequencing of DNA” (Part# 111257047 Rev. A) using 25-50 ng of input genomic DNA or 5hmC-captured DNA to initiate the protocol [PMID: 21151123]. Enriched DNA from MeDIP were subjected to library construction using the NEBNext ChIP-Seq Library Prep Reagent Set for Illumina according the manufacturer’s protocol.