Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD9

Cell type

Cell type Class
Blood
Cell type
NOMO-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia

Attributes by original data submitter

Sample

source_name
NOMO-1
cell type
Acute myeloid leukemia
genotype
MLL-AF9/KrasG13D
treatment
none
antibody
BRD9 (A303-781A; Bethyl)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with appopriate sizes were prepared for sequencing. These libraries were made following the instructions of illumina Truseq DNA sample prep kit v2.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
91061673
Reads aligned (%)
85.4
Duplicates removed (%)
12.1
Number of peaks
5546 (qval < 1E-05)

hg38

Number of total reads
91061673
Reads aligned (%)
88.8
Duplicates removed (%)
9.7
Number of peaks
6205 (qval < 1E-05)

Base call quality data from DBCLS SRA